Clemson Translational Research Imaging Core (CTRIC)
Our projects are supported by state-of-the-art core facilities in Clemson, Greenville, and Charleston. CTRIC is lead by core director: Dan Simionescu, a Jerry E. and Harriet Calvert Dempsey Professor, and core co-directors: Terri Bruce, Research Assistant Professor and Clemson Light Imaging Facility Director and Russell Norris, Associate Professor at Regenerative Medicine Department at MUSC.
CORE DIRECTOR
CO-DIRECTOR
CO-DIRECTOR
Project Overview
The Clemson Translational Research Imaging Core (CTRIC) is supported by two thrusts and serves the needs of our COBRE projects by serving as a portal to expertise, training, and mentoring in the following areas: preclinical validation of regenerative medicine approaches, animal models, surgery and bioimaging, utilization of stem cells, molecular biology, histology, and advanced microscopical imaging and biophotonics. The Core provides all the necessary support to develop ideas, overcome technical challenges, and lead projects toward significant outcomes. CTRIC labs, facilities, and expertise are available to all previous and current COBRE scientists and students as well to all SC specialists from academia, medical research institutions, and industry. Services are provided by two individual thrusts.
Focus Areas
Thrust I: Bio-Analytical
- Stem Cell Biology: Stem cell isolation, Stem cell differentiation, Cell phenotyping
- Advanced imaging: Light, polarized microscopy, Fluorescent microscopy, Electron microscopy
- Advanced Histology: Tissue processing, Classical/special histology, Immunostaining
- Molecular biology: Protein expression, Gene expression, Gene transfection, silencing
- Digital Image Processing: 3D reconstruction
Thrust II: Pre-Clinical Validation Thrust
- Consultations: Animal models, Grant proposals, IACUC protocols
- Animal care: Pre-operative care, Post-operative care
- In Vivo Imaging: Ultrasound, Doppler, MicroCT, IVIS Lumina XR
- Technical Services: Surgical assistance, Euthanasia, Blood, sample collection
- Clinical imaging: Large animal MRI, Large animal CT
| Thrust | Facilities and Locations | Leader and Support Personnel | Services |
|---|---|---|---|
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I. Bio-Analytical |
Regenerative Medicine and Stem Cell Laboratory at Greenville and Clemson |
Stem cell biology and differentiation, cell sorting and in vivo tracking |
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Light Imaging and Electron Microscopy Facility at Clemson and Charleston |
Confocal, hyperspectral imaging, FACS, TEM, SEM, 3D cell and tissue imaging |
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II. Pre-Clinical Validation |
Godley Snell Research Center and Experimental Farm at Clemson |
Development and oversight of IACUC protocols, small animal model studies, bioimaging |
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Godley Snell Research Center, Experimental Farm at Clemson, AVS Image Analysis Laboratory |
Development and oversight of IACUC protocols, large animal model studies, clinical bioimaging |
View a list of instruments in the core. Additional information about complete instrumentation and services provided through the Clemson Light Imaging Facility (CLIF), Clemson Electron Microscopy, and Godley Snell Research Center
For more information about our core facilities, services, and consultations please contact our core director, Dr. Simionescu, or lab manager Dr. Nagy Mehesz.
CTRIC Core Billing Rates
| CTRIC Core Billing Rates, FY 22/23 | |||
| Description of Commodity or Service | Unit of Billing | Internal Rates (Academia) |
External Rates (Corporate) |
|---|---|---|---|
| Cell culture room (sterile room with all utilities for cell culture work) | per hour | $ 15.00 | $ 30.00 |
| Classical histology staining (supplies included) | per slide | $ 5.00 | $ 10.00 |
| Frozen embedding (supplies included) | per hour | $ 20.00 | $ 40.00 |
| Frozen sectioning (cryotome)(supplies included) | per hour | $ 20.00 | $ 40.00 |
| Flow Cytometry (Attune NxT) | per hour | $ 50.00 | $ 100.00 |
| Fluorescent microscopy, Keyence BZ-X810 | per hour | $ 15.00 | $ 30.00 |
| H&E Staining | per hour | $ 1.00 | $ 3.00 |
| Immunohistochemistry (supplies, but not primary antibody included) | per slide | $ 7.00 | $ 20.00 |
| Paraffin embedding (embedding station) (supplies included) | per hour | $ 15.00 | $ 30.00 |
| Paraffin sectioning (microtome) Supplies included | per hour | $ 15.00 | $ 30.00 |
| qPCR, Reverse Transcription (Rotor gene Q) | per hour | $ 30.00 | $ 60.00 |
| Western Blot, automated (Jess) | per one run | $ 130.00 | $ 300.00 |
| Tissue Processing for paraffin embedding (tissue processor) (supplies included) | per one run | $ 30.00 | $ 60.00 |
| Consultation | per hour | n/a | $ 100.00 |
| Technical Assistance | per hour | n/a | $ 100.00 |